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15/06/2007 · Leaves were placed in a pressure chamber and pressurized to MPa. Pressurization took place over 5–10 s to avoid excessive (>35 °C) increases in leaf temperature. Exudation from the petiole was collected into preweighed Eppendorf tubes ( mL) at intervals of 30 s during the initial phase, and subsequently at 60 s intervals.
For each sample 300 mg of crushed frozen leaves is weighed in a precooled 2 mL plastic tube (Eppendorf). These tubes can also be stored at 80 ˚C for a few days before use. Care is taken to prevent thawing of the frozen material up until this point, by precooling of all materials which comes in contact with the plant powder.
Rom et al. (1993), whereby 300 mg leaf discs (6 mm in diameter) were removed from top leaves, ground and reconstituted in 750 |il of N NaOH in Eppendorf tubes. The contents were then centrifuged at 10,000 g for 2 min, and the supernatant diluted with N NaOH to the desired concentrations. The third extraction technique was performed
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29/01/2010 · Fresh leaf samples were ground in a ball mill with 3 balls in 2 ml Eppendorf tubes for 30 s at a speed of 32 s1. Grinding beakers were precooled at 18°C. Solvent was added afterwards. ... a homogeneous crushing of leaf material was obtained using the handheld disperser. Leaves were shredded with this equipment to a maximum particle size of ...
08/05/2018 · The E. umbellatus leaf sample was weighed on an analytical balance in a Eppendorf tube and then crushed in the same tube with a microspatula for 0, 15, 30, 60, or 180 s. Samples were prepared in triplie.
14/08/2020 · Results showed that the optimal condition was 10 mg of leaf extracted using nhexane in an ultrasonior with ultrasonic power of 80 W for 1 min. Furthermore, the absolute content of S(+)linalool obtained by UAME ( ± mg/g leaf) was comparable with that extracted by the 30min hydrodistillation (HD) ( ± mg/g leaf).
04/10/2012 · Pack the leaves from each plant (or small pool, if carrying out a mutant screen; Figs. 3 and 5a) into a 2ml Eppendorf tube. Freeze the leaves in liquid nitrogen and break them using a plastic ...
1. Freshleaf tissue ( g) was ground in a Eppendorf tube with a micropestle and preheated freshly prepared 800 µL extraction buffer was immediately added to the tube. 2. Seeds, sundried and shadedried leaf samples ( g) were grounded to fine powder with a pestle and mortar and transferred to a Eppendorf tube and 800 µL
Ethanolic Leaves Extract, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS scores, article reviews, protocol conditions and more
One hundred μL of each dilution was then inoculated on Tryptone Soya Agar, TSA (Oxoid, United Kingdom) in triplies under aseptic leaf in eppendorf using vortex gently crush the tissue clumps into small pieces with the pestle. from the mortar into a pre chilled, 2 ml eppendorf tube kept frozen in an .
leaves were shade dried. The dried leaves were crushed and stirred in petroleum ether for 30 min. The extract was separated from debris by filtration using a sterile muslin cloth. The procedure was repeated twice and the filtrate obtained was centrifuged. The supernatant was pooled and then using .
The leaves are entire and arranged in pairs and opposite each other along the stem. The leaves ... fresh Gomphocarpus leaves were crushed in liquid nitrogen to a finer powder using a mortar and pestle. The samples were then placed in eppendorf tubes. While working with this fresh tissue, it
The sample solution was prepared from dried R. matsumuranus leaves (50 mg) and 5 mL of methanol in an Eppendorf tube. The mixture was sonied for 30 min at 50 °C (ultrasound power 100 W, frequency 35 kHz), centrifuged (6000× g ), filtered (using PTFE syringe filter), and transferred to the volumetric flask (10 mL), and the final ...
Preparation of Plant Extract: Shade dried leaves of E. alba crushed into powdered form. The powdered material was extracted in 3different solvents of increasing polarity. 10 mg of dried leaf powder was extracted by cold percolation method separately in 100 ml of Petroleum ether, Acetone, and Ethanol respectively for 24 h.
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