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Western blot AntiTPH2 antibody (ab125877) All lanes : AntiTPH2 antibody (ab125877) at 1/1000 dilution. Lane 1 : IMR32 whole cell lysate. Lane 2 : U87MG whole cell lysate. Lysates/proteins at 30 µg per lane. Predicted band size: 56 kDa. 10% SDS PAGE.
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Immunoprecipitation AntiTPH2 antibody (ab111828) Immunoprecipitation analysis of Raphe Nuclei isolated from SpragueDawley, precipitating ab111828 coupled to magnetic beads, which was introduced to whole cell extracts at room temperature for 15 mins. Immunoprecipitated proteins were assessed by Western blotting.
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09/01/2020 · Stability. The stability of the TPH in different conditions was examined using the protocol described in reference [].Cell culture. Human lung adenocarcinoma A549 cells, drugresistant A549 cells overexpressing Pgp (A549/ADR), and drugresistant mouse breast cancer 4T1 (4T1/ADR) cells (College of Pharmaceutical Science, Guangzhou Medical University, .
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